RNA-Mediated RNA Degradation and Chalcone Synthase A Silencing in Petunia
Identifieur interne : 003D30 ( Main/Exploration ); précédent : 003D29; suivant : 003D31RNA-Mediated RNA Degradation and Chalcone Synthase A Silencing in Petunia
Auteurs : M. Metzlaff [Royaume-Uni] ; M. O'Dell [Royaume-Uni] ; P. D Cluster [Royaume-Uni] ; R. B Flavell [Royaume-Uni]Source :
- Cell [ 0092-8674 ] ; 1997.
English descriptors
- Teeft :
- Aliquot, Amplification, Annealing, Boehringer mannheim, Cdna, Chalcone, Chalcone synthase, Chsa, Chsa coding region, Chsa endogene, Chsa rnas, Cleavage, Cleavage site, Coding region, Complementarity, Complementary sequences, Cosuppression, Degradation, Endogene, Endogene chsa, Endonucleolytic, Endonucleolytic cleavage, Endonucleolytic cleavages, Floral cosuppression, Flower tissue, Flower tissues, Fragment, High levels, Hybrida, Jorgensen, Leaf tissue, Mrna, Napoli, Nontransgenic, Oligonucleotide, Pairing, Petunia, Petunia hybrida, Plant cell, Posttranscriptional, Posttranscriptional chsa, Primer, Purple flower tissue, Purple flowers, Rna, Rnase, Sequencing, Synthase, Transgene, Transgene chsa, Transgenic, Transgenic lines, Transgenic plants, White flower tissue, White sectors.
Abstract
Abstract: Transgenic Petunia plants with a chsA coding sequence under the control of a 35S promoter sometimes lose endogene and transgene chalcone synthase activity and purple flower pigment through posttranscriptional chsA RNA degradation. In these plants, shorter poly(A)+ and poly(A)− chsA RNAs are found, and a 3′ end–specific RNA fragment from the endogene is more resistant to degradation. The termini of this RNA fragment are located in a region of complementarity between the chsA 3′ coding region and its 3′ untranslated region. Equivalent chsA RNA fragments remain in the white flower tissue of a nontransgenic Petunia variety. We present a model involving cycles of RNA–RNA pairing between complementary sequences followed by endonucleolytic RNA cleavages to describe how RNA degradation is likely to be promoted.
Url:
DOI: 10.1016/S0092-8674(00)81930-3
Affiliations:
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Le document en format XML
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D Cluster</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH</wicri:regionArea><wicri:noRegion>Norwich NR4 7UH</wicri:noRegion></affiliation></author><author><name sortKey="Flavell, R B" sort="Flavell, R B" uniqKey="Flavell R" first="R. B" last="Flavell">R. B Flavell</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH</wicri:regionArea><wicri:noRegion>Norwich NR4 7UH</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Cell</title><title level="j" type="abbrev">CELL</title><idno type="ISSN">0092-8674</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1997">1997</date><biblScope unit="volume">88</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="845">845</biblScope><biblScope unit="page" to="854">854</biblScope></imprint><idno type="ISSN">0092-8674</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0092-8674</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Aliquot</term><term>Amplification</term><term>Annealing</term><term>Boehringer mannheim</term><term>Cdna</term><term>Chalcone</term><term>Chalcone synthase</term><term>Chsa</term><term>Chsa coding region</term><term>Chsa endogene</term><term>Chsa rnas</term><term>Cleavage</term><term>Cleavage site</term><term>Coding region</term><term>Complementarity</term><term>Complementary sequences</term><term>Cosuppression</term><term>Degradation</term><term>Endogene</term><term>Endogene chsa</term><term>Endonucleolytic</term><term>Endonucleolytic cleavage</term><term>Endonucleolytic cleavages</term><term>Floral cosuppression</term><term>Flower tissue</term><term>Flower tissues</term><term>Fragment</term><term>High levels</term><term>Hybrida</term><term>Jorgensen</term><term>Leaf tissue</term><term>Mrna</term><term>Napoli</term><term>Nontransgenic</term><term>Oligonucleotide</term><term>Pairing</term><term>Petunia</term><term>Petunia hybrida</term><term>Plant cell</term><term>Posttranscriptional</term><term>Posttranscriptional chsa</term><term>Primer</term><term>Purple flower tissue</term><term>Purple flowers</term><term>Rna</term><term>Rnase</term><term>Sequencing</term><term>Synthase</term><term>Transgene</term><term>Transgene chsa</term><term>Transgenic</term><term>Transgenic lines</term><term>Transgenic plants</term><term>White flower tissue</term><term>White sectors</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Transgenic Petunia plants with a chsA coding sequence under the control of a 35S promoter sometimes lose endogene and transgene chalcone synthase activity and purple flower pigment through posttranscriptional chsA RNA degradation. In these plants, shorter poly(A)+ and poly(A)− chsA RNAs are found, and a 3′ end–specific RNA fragment from the endogene is more resistant to degradation. The termini of this RNA fragment are located in a region of complementarity between the chsA 3′ coding region and its 3′ untranslated region. Equivalent chsA RNA fragments remain in the white flower tissue of a nontransgenic Petunia variety. We present a model involving cycles of RNA–RNA pairing between complementary sequences followed by endonucleolytic RNA cleavages to describe how RNA degradation is likely to be promoted.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Metzlaff, M" sort="Metzlaff, M" uniqKey="Metzlaff M" first="M" last="Metzlaff">M. Metzlaff</name></noRegion><name sortKey="Cluster, P D" sort="Cluster, P D" uniqKey="Cluster P" first="P. D" last="Cluster">P. D Cluster</name><name sortKey="Flavell, R B" sort="Flavell, R B" uniqKey="Flavell R" first="R. B" last="Flavell">R. B Flavell</name><name sortKey="O Dell, M" sort="O Dell, M" uniqKey="O Dell M" first="M" last="O'Dell">M. O'Dell</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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